Thanks for the benchmarks, ux-3. So if I may summarize the conclusions from your numbers:
It looks like there is a small but acceptable slowdown when you install a USB 2.0 PCI card in an older machine, but there will be considerable slowdown once you plug in any high-speed devices, even if you don't actually use to them. So only plug in high-speed devices as needed. And also, there is an additional (albeit small) further slowdown if you plug any low speed (or so-called "full speed") devices such as keyboards and mice into the USB ports on to USB 2.0 card, so it's best to keep those plugged into the integrated USB 1.x ports of the system, if possible.
Considering how the bandwidth of the 33MHz Legacy PCI bus is 133 megaBYTES per second, which is 1064 megaBITS per second, then the maximum bandwidth of USB 2.0 (EHCI), being 480 megabits per second, would potentially take up almost half of the PCI bus bandwidth. So considerable slowdown is not surprising when high speed USB 2.0 devices are used. I guess the USB controller needs to "reserve" some of that PCI bandwidth even when a connected high speed USB 2.0 device is idle.
I've asked this in this thread before, but I'm really curious what would happen to system performance if a USB 3.0 (XHCI) card were to be installed on such an old system, through use of a PCIe to Legacy PCI adapter. With a theoretical maximum bandwidth of 5 gigabits per second, such a card could potentially hog the entire bandwidth of the Legacy PCI bus. Would such a system still be usable? And how would bandwidth be divided between contending devices?
It would require the installation of a newer Windows or Linux version, but it would be a really fun and interesting experiment, I think.
Also, I'm curious about what the performance impacts would be with a motherboard with an AGP graphics card, since AGP cards would get their own dedicated bandwidth that they wouldn't have to share with any cards on the PCI bus. In such setups, you'd think that only disk perfomance would be affected by the addition of a USB 2.0 card, and graphics performance would remain unaffected.